Table 8.

K_m and V_max values of the enzyme determined by Lineweaver–Burk and Woolf–Hanes plots for different substrates.

Substrate	Km	Vmax	Vmax/Km
Lineweaver–Burk plot
4-Nitrophenyl butyrate	0.51 mM	16.98 U/mg	33.29
4-Nitrophenyl laurate	0.15 mM	15.44 U/mg	99.81
4-Nitrophenyl myristate	0.17 mM	14.61 U/mg	102.93
4-Nitrophenyl palmitate	0.19 mM	13.59 U/mg	71.53
Woolf–Hanes plot
4-Nitrophenyl butyrate	0.54 mM	17.45 U/mg	32.31
4-Nitrophenyl laurate	0.13 mM	15.13 U/mg	116.38
4-Nitrophenyl myristate	0.18 mM	14.94 U/mg	85.94
4-Nitrophenyl palmitate	0.17 mM	13.45 U/mg	79.11