Table 2.
Carbohydrate specificity of Fusarium spp. lectins.
| Carbohydrate | F. fujikuroi | F. beomiformii | F. begoniae | F. nisikadoi | F. anthophilum | F. tabacinum | F. incarnatum |
|---|---|---|---|---|---|---|---|
| d-Ribose | – | – | 25 mM | 3.12 mM | 12.5 mM | 6.25 mM | 6.25 mM |
| l-Rhamnose | – | – | 3.12 mM | 6.25 mM | 3.12 mM | – | – |
| Xylose | – | – | 50 mM | 3.12 mM | 25 mM | – | – |
| l-Fucose | – | – | 12.5 mM | 3.12 mM | 12.5 mM | – | 12.5 mM |
| d-Glucose | – | – | 50 mM | 1.56 mM | 3.12 mM | – | 3.12 mM |
| d-Mannose | – | – | – | 25 mM | – | – | – |
| d-Arabinose | – | – | – | 25 mM | 12.5 mM | – | 12.5 mM |
| l-Arabinose | – | – | 12.5 mM | 12.5 mM | 50 mM | – | 50 mM |
| d-Galactose | – | – | 12.5 mM | – | – | – | 50 mM |
| d-Fructose | – | – | 1.56 mM | – | – | 50 mM | 12.5 mM |
| d-Mannitol | – | – | 3.12 mM | – | 50 mM | 0.78 mM | 25 mM |
| d-Sucrose | – | – | 12.5 mM | – | 6.25 mM | – | – |
| d-Maltose | – | – | 3.12 mM | – | 1.56 mM | – | – |
| d-Lactose | – | 3.12 mM | 50 mM | – | – | – | – |
| Melibiose | – | – | – | – | – | – | – |
| d-Trehalose dehydrate | – | – | 1.56 mM | – | – | – | – |
| d-Raffinose | – | – | 3.12 mM | – | – | 3.12 mM | 0.78 mM |
| Maltotriose | – | – | 12.5 mM | – | 12.5 mM | – | – |
| Inositol | – | – | 12.5 mM | – | – | – | 12.5 mM |
| Meso-inositol | – | – | 6.25 mM | – | – | – | 25 mM |
| d-Glucosamine hydrochloride | – | – | 25 mM | 6.25 mM | – | – | – |
| d-Galactosamine hydrochloride | 3.12 mM | 12.5 mM | 25 mM | 12.5 mM | 6.25 mM | – | 6.25 mM |
| d-Glucuronic acid | – | – | 12.5 mM | – | 3.12 mM | – | 25 mM |
| d-Galacturonic acid | 1.56 mM | 1.56 mM | 6.25 mM | – | 50 mM | – | 50 mM |
| N-acetyl-d-glucosamine | – | – | 12.5 mM | 25 mM | – | – | – |
| N-acetyl-d-galactosamine | – | 12.5 mM | 6.25 mM | 25 mM | 12.5 mM | – | 12.5 mM |
| 2-Deoxy-d-glucose | – | – | 25 mM | 12.5 mM | – | 250 μg/mL | – |
| 2-Deoxy-d-ribose | – | – | 25 mM | 125 μg/ml | 25 mM | – | 12.5 mM |
| N-acetyl neuraminic acid | – | 12.5 mM | 0.195 mM | 12.5 mM | 3.90 mM | – | – |
| N-glycolyl neuraminic acid | – | – | – | – | – | – | – |
| Bovine submaxillary mucin | 31.25 μg/mL | 0.975 μg/mL | 0.06 μg/mL | 0.487 μg/mL | 0.12 μg/mL | 62.5 μg/mL | 31.25 μg/mL |
| Porcine stomach mucin | – | – | 0.975 μg/mL | – | 0.975 μg/mL | – | – |
| Fetuin | 125 μg/mL | 500 μg/mL | 0.243 μg/mL | – | 0.121 μg/mL | 500 μg/mL | – |
| Asialofetuin | 250 μg/mL | 250 μg/mL | 0.487 μg/mL | 125 μg/mL | 0.975 μg/mL | – | 31.25 μg/mL |
| Chondroitin-6-sulphate | – | – | 500 μg/mL | – | 15.62 μg/mL | – | 31.25 μg/mL |
| Inulin | – | – | 125 μg/mL | – | 125 μg/mL | – | 500 μg/mL |
| Pullulan | – | – | 500 μg/mL | – | – | 15.62 μg/mL | 62.5 μg/mL |
| Starch | 1.95 μg/mL | – | 500 μg/mL | – | 3.90 μg/mL | – | – |
Note: –, non-inhibitory carbohydrates.
Simple sugars were tested at a final concentration of 100 mM, while glycoproteins and polysaccharides were tested at a concentration of 1 mg/mL. The test solutions were as two-fold serially diluted across the wells of microtiter plates and incubated with appropriately diluted lectin to determine minimum inhibitory concentration of specific carbohydrate.