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. 2016 May 23;(111):53979. doi: 10.3791/53979
Reaction Components Number of reactions
2 5
PBS 875 µl 2.19 ml
Copper protectant  20 µl 50 µl
Fluorescent dye picolyl azide  5 µl 12.5 µl
Diluted reaction buffer prepared in 4.1  100 µl 250 µl
Total reaction volume 1 ml 2.5 ml