Skip to main content
. 2016 May 26;(111):53431. doi: 10.3791/53431
Section Name of Equipment Company Cat. No. Notes Images
2 Glass HFB Module Soham Scientific  ---  Custom Item.
2.1 Sigmacote® Sigma-Aldrich SL2
2.3 Silicoset 151 Intertronics ACCSS151 Silicone Glue.
2.5 PTFE tape Sigma-Aldrich Z104388
3.2.1 Reservoir bottle Fisher 11972619
Q-series cap Kinesis 00932Q-3V PTFE the screw threads of the adapters and fitting nut. Attach to the Q-series cap. Attach an 8.5 cm section of the supplied PTFE tubing under the 1 mm adapter and a 4 cm section under the 3 mm adapter. graphic file with name jove-111-53431-7.jpg
Adapter, Male, 1.0 mm ID Kinesis 008NB10-KD5L
Adapter, Male, 3.0 mm ID Kinesis 008NB30-KD5L
Fitting Nut Kinesis U-350
Neoprene tubing Fisher 10366344 Attach the Hepa filter to 6 cm of neoprene tubing and attach this to the 'fitting nut'. Attach a 2x 30 mm sections of L/S14 tubing to the top two barbs of the Y-connector and a 3 cm section of L/S16 tubing to the bottom. Attach this to the barb of the 3 mm ID adapter. (Section 3.2.1) graphic file with name jove-111-53431-8.jpg
HEPA-vent Fisher 11374634
Y-connector, barbed Cole Parmer OU-06295-10
L/S16 Silicone tubing Cole Parmer OU-96410-16
L/S14 Silicone tubing Cole Parmer WZ-96410-14
L/S13 Silicone tubing Cole Parmer OU-96410-13 80 cm to connect the 1.0 mm barbed adapter on the Q-series cap to the pump tubing = Feed tube.
WM 205U/CA pump Fisher 1248-6300
WM pump tubing, PVC, blue-orange, 0.25 mm bore Fisher 12416310 PTFE the screw thread of the male adapter and connect the female adapter. Work the pump tubing over one of the barbs. Repeat this set-up at the other end of the tubing. (Section 3.2.1) graphic file with name jove-111-53431-9.jpg
Adapter, Male, 1.0 mm ID Kinesis 008NB10-KD5L
Adapter, Female, 1.0 mm ID Kinesis 008NB10-KD2L
3.2.2 Female Luer cap Cole Parmer WZ-45508-64 Side port end caps. graphic file with name jove-111-53431-10.jpg graphic file with name jove-111-53431-11.jpg
L/S13 Silicone tubing Cole Parmer OU-96410-13 40 mm section to connect the pump tubing to a module connector.
L/S16 Silicone tubing Cole Parmer OU-96410-16 3x 30 mm of L/S16 fitted to 3x reducers = module connectors. (Section 3.2.2)
Barbed reducer 1/8" x 1/16" Cole Parmer 30616-43
3.2.4 L/S13 Silicone tubing Cole Parmer OU-96410-13 55 cm section to connect the retentate to the L/S14 of the Y-connector on the Q-series cap. graphic file with name jove-111-53431-12.jpg
L/S13 Silicone tubing Cole Parmer OU-96410-13 45 cm section to connect the permeate to the L/S14 of the Y-connector on the Q-series cap.
Straight barbed union Cole Parmer WZ-30612-43 Attach to the end of the L/S13 that will connect with the L/S14 of the Y-connector.
3.4.2 Clamp VWR 229-0609
4.3 4 mm Silicone tubing  Fisher FB68858 Fold over a 40 mm section of tubing and secure with a cable tie = Module end cap. (Section 4.3) graphic file with name jove-111-53431-13.jpg
Cable tie Fisher 12326377
4.4 MACSmix tube rotator Miltenyi Biotech 130-090-753 An adaptation may be required to attach the modules.
4.5 Leur Injection port Thistle Scientific IB-10820 Attach the end cap to the injection port. (Section 4.5)
Female Luer cap Cole Parmer WZ-45508-64
5 L-lactic acid kit Megazyme K-LATE
5 D-glucose kit Megazyme K-GLUC
6.2 Scalpel / micro knife InterFocus 10315-12
7.4.3 Albumin ELISA Bethyl Labs E80-129