| PYG composition | quantities |
| Proteose peptone | 20 g |
| Yeast extract | 2 g |
| MgSO4. 7H2O | 0.980 g |
| CaCl2 | 0.059 g |
| citrate sodium. Dihydrate | 1 g |
| Fe(NH4) 2(SO4) 2 x 6 H2O | 0.02 g |
| Glucose | 18 g |
| distilled water for | 1 L |
| Adjust pH at 6.8 with HCl or KOH. | |
| Autoclave 15 min at 121 °C. | |
| Starvation medium | quantities |
| Yeast extract | 2 g |
| Glucose | 18 g |
| Fe(NH4) 2(SO4) 2 . 6 H2O | 0.02 g |
| PAS (detailed below) | 1 L |
| Filtered on 0.22 mm | |
| PAS solution A | quantities |
| KH2PO4 | 0.136 g |
| Na2HPO4 | 0.142 g |
| PAS solution B | quantities |
| MgSO4.7H2O | 4.0 mg |
| CaCl2.2H2O | 4.0 mg |
| NaCl | 0.120 g |
| 10 ml of each solution A and B, are added into 1 L of distilled water. |
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