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. 2016 Jun 7;(112):53866. doi: 10.3791/53866
Paraventricular Nucleous of the Hypothalamus
Section Target Density-fmol/g Scan Area  Total Target Area Total*
Total 1 996 4,383 4,383 996
Total 2 921 3,362 3,362 921
Total 3 818 3,445 3,445 818 Specific Binding
Total 4 710 2,906 2,906 710 967
AVERAGE 861 3,524 3,524 861 895
763
Section Target Density-fmol/g Scan Area  Total Target Area Non-specific* 678
NSP 1 53 3,072 1,737 30 826
NSP 2 52 2,959 1,455 26
NSP 3 86 3,180 2,035 55
NSP 4 53 3,293 1,995 32
AVERAGE 61 3,126 1,805.5 36
Bed Nucleus of the Accessory Olfactory Tract
Section Target Dens-fmol/g Scan Area  Total Target Area Total*
Total 1 439 3,632 3,632 439
Total 2 435 3,632 3,632 435
Total 3 355 3,632 3,620 354
Total 4 435 3,632 3,631 435 Specific Binding
Total 5 342 3,632 3,630 342 414
Total 6 334 3,632 3,606 331 393
AVERAGE 390 3,632 3,625 389 321
394
315
Section Target Dens-fmol/g Scan Area  Total Target Area Non-specific* 320
NSP 1 49 3,632 1,846 25 360
NSP 2 64 3,632 2,362 42
NSP 3 53 3,632 2,275 33
NSP 4 64 3,632 2,339 41
NSP 5 51 3,632 1,879 26
NSP 6 41 3,632 966 11
AVERAGE 54 3,632 1,945 30
*Total and Non-speciic are  density units of fmol/g.
Total = density * total target area/scan area
Non-specific = density * total target area/scan area
Total Target Area is pixels that exceeded the threshold value, set as close to 0.00 fmol/g as possible.
Pixels not exceeding the threshold value receive a value of 0 for assessment of total density. 
Pixels not exceeding the threshold value receive a value of 0 for assessment of Non-specific density. 
Specific Binding is Total minus Non-specific binding expressed as density units of fmol/g.