| Reagent | Amount |
| 2x Master Mix | 10 μl |
| Forward primer (10 μM) | 0.8 μl |
| Reverse primer (10 μM) | 0.8 μl |
| Probe (10 μM) | 0.4 μl |
| Water | 7.0 μl |
| Final volume | 19 μl |
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