| Descriptor | Significance |
| Correlation coefficient (R2) | A measure of the linearity of the standard curve. Ideally it should be R2=1. Value of R2=0.98-0.99 are acceptable. |
| Slope (α) | A measure of reaction efficiency. Ideally it should be equivalent to -3.32. Value in the range between -3.58 and -3.1. |
| Efficiency (=10(-1/slope)-1) | If it is 100%, the templates doubles after each thermal cycle during exponential amplification. A good efficiency range is between 90 and 110%. |
| Y Intercept (β) | The theoretical limit of detection of the reaction. Although not used to quantify reaction sensitivity, it is important when comparing different standard curves of the same target. |
Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.