Reagent	Amount
Undiluted RNA / 1:10 diluted RNA	1.0 µl
10x PCR Buffer	5.0 µl
dNTPs 10 mM	1.0  µl
63F forward primer	1.0 µl
CAG GCC TAA CAC ATG CAA GTC
518R reverse primer	1.0 µl
ATT ACC GCG GCT GCT GG
Taq polymerase	0.4 µl
RNAse-free dH2O	40.6 µl