Fig.1.
Experimental overview. Mice were randomly assigned to be mock-immunized (mock) or immunized. All mice received three rounds of subcutaneous injections in the groin with a 3-week interval. Mock mice received mock vaccine, immunized mice received our trivalent vaccine. Blood samples were collected from the tail vein and the sera was analyzed by ELISA. Based on the anti-oligomeric Aβ1-42 endpoint titers obtained (ELISA 1), eight well-responding immunized mice and three mock mice were selected for oligomeric Aβ1-42-induced NBM lesion surgery (group A). Remaining mice received a fourth immunization and another tail puncture. Based on the titers obtained (ELISA 2),eight immunized and four mock mice were selected for NBM lesion surgery (group B). Remaining mice (group C: eight immunized, two mock mice) received a fifth immunization before NBM lesion surgery. Mice from each group were transcardially perfused ten days after NBM lesion surgery. Final blood samples were collected from the heart just before perfusion and sera obtained were analyzed later (ELISA 3).Experimental days are indicated on the left.