Fig.2.

Average of the ¹⁰log value of anti-oligomeric Aβ_1-42 ELISA endpoint titers of immunized and mock mice before and after Aβ_1-42-induced NBM lesions. After all mice had received three (mock-) immunizations, mice with high titers (ELISA 1) and randomly selected mock mice were selected as the first group to receive NBM lesion surgery (group A). Remaining mice received a fourth (mock-) immunization. Again, mice with high titers (ELISA 2) and randomly selected mock mice were selected as the second group to receive NBM lesion surgery (group B). Remaining mice (group C) received a fifth (mock-) immunization before lesion surgery. Measurements before NBM lesions (ELISA 1 and 2) were performed on sera from blood samples collected from the tail vein. Measurements after NBM lesions (ELISA 3) were performed on sera from blood samples taken from the heart, just before transcardial perfusion. The results show that three rounds of immunizations are highly effective, while a fourth and fifth round of immunizations have only minor added effect. Vertical axis categories are numbered between square brackets for easy reference. Error bars represent SEM. Statistical indicators: ^*p < 0.05, ^***p < 0.001, ns indicates p > 0.05.