Fig.3.
Active immunization prevents the loss of cholinergic innervations induced by oligomeric Aβ1-42 injected into the NBM. Shown are representative images of ChAT-positive fibers in the parietal neocortex of mock (A and B) and immunized mice (C and D). Images on the left (A and C) show the side of the brain injected with scrambled Aβ1-42 control peptide (Aβ-scr). Images on the right (B and D) show the contralateral lesion-sides of the brain injected with oligomeric Aβ1-42 (Aβ-oligo). Note that in practice the compounds were randomly injected in the left- or right side of the brain. Comparing control and lesion sides of the brain, immunized mice show reduced fiber loss compared to mock mice. In each image the area between the parallel bars indicate the quantified area (layer V of the somatosensory cortex). The horizontal scale bar shown in (D) applies to all images and represents 100 μm.