Fig.2.

PDE5 is expressed in neurons. Immunohistochemistry was performed on formalin-fixed, paraffin embedded sections of cortex (A1-A3), hippocampus (B1-B3; shown is subfield CA2/3 – see main text for details) and cerebellum (C1-C3). All images are shown at 200x magnification; the scale bar in Panel A1 applies to all panels. Panels A1, B1, and C1 use an Abcam antibody, panels A2, B2, and C2 use a Santa Cruz antibody, and panels A3, B3, and C3 use an Atlas antibody (see Methods). In all cases, PDE5 is expressed in the cytoplasm of neurons. In cortex and hippocampus, PDE5 is expressed primarily in large, pyramidal-type neurons (see arrows), whereas in cerebellum, it is prominent in Purkinje neurons (see arrows).