Fig.2.

p5-WT peptide concentration-dependent effects on the phosphotransferase activities of CDK5, ERK1 and GSK3β. IC₅₀ determination for compound p5-WT against protein kinases. A graph of log inhibitor versus normalized response with variable slope was generated using the Prism software. The graph showed increased inhibition of the phosphotransferase activities of CDK5 and ERK1, and increased stimulation of GSK3β with increasing compound concentration. The IC₅₀ value for compound p5-WT against CDK5/p25 was determined to be 169 μM, the IC₅₀ value for compound p5-WT against CDK5/p35 was determined to be 217 μM, and the IC₅₀ value for compound p5-WT against ERK1 was determined to be 1322 μM.