Table 1.

% Activity of CDK5/p25 in the presence of inhibitory peptides using radiometric assay method arranged from lowest to highest counts (cpm), with two independent experiments in duplicates. Each assay solution contained 10% DMSO; The blank control without histone was 1,217 cpm, and with histone was 202,940 cpm. The cpm values given in the table was subtracted for the blank with histone in the presence of different peptides. The cpm value in the presence of the p5-WT peptide (#1) was taken as 100% phosphotransferase activity. The negative numbers in the percent change from control (% CFC) values correspond to even greater inhibition and positive numbers are less inhibition in the CDK5 phosphotransferase activity relative to the p5- WT peptide

Peptide ID	Peptide Sequence	CPM	% Activity	% CFC
78	KEAFWDRCLSVINLMSSKMLQINM	89762	74	–26
77	KEAFWDRCLSVINLMSSKMLQINV	90305	75	–25
15	KEAFYDRCLSVINLMSSKMLQINA	111665	92	–8
76	KEAFWDRCLSVINLMSSKMLQINL	113022	93	–7
1	KEAFWDRCLSVINLMSSKMLQINA	121167	100	0
20	KEAFWDRALSVINLMSSKMLQINA	121639	100	0
4	KNAFWDRCLSVINLMSSKMLQINA	122973	101	1
33	KEAFWDRCLSIINLMSSKMLQINA	123757	102	2
48	KEAFWDRCLSVINLMTSKMLQINA	126451	104	4
79	KEAFWDRCLSVINLMSSKMLQINS	131811	109	9
16	KEAFWERCLSVINLMSSKMLQINA	135248	112	12
75	KEAFWDRCLSVINLMSSKMLQINI	138095	114	14
14	KEAFFDRCLSVINLMSSKMLQINA	141068	116	16
29	KEAFWDRCLMVINLMSSKMLQINA	141207	117	17
28	KEAFWDRCLYVINLMSSKMLQINA	141312	117	17
61	KEAFWDRCLSVINLMSSKMVQINA	141773	117	17
41	KEAFWDRCLSVINIMSSKMLQINA	142416	118	18
42	KEAFWDRCLSVINVMSSKMLQINA	145310	120	20
3	KDAFWDRCLSVINLMSSKMLQINA	145721	120	20
18	KEAFWNRCLSVINLMSSKMLQINA	149428	123	23
59	KEAFWDRCLSVINLMSSKCLQINA	150317	124	24
17	KEAFWQRCLSVINLMSSKMLQINA	151097	125	25
34	KEAFWDRCLSVLNLMSSKMLQINA	151937	125	25
13	KEAWWDRCLSVINLMSSKMLQINA	152154	126	26
53	KEAFWDRCLSVINLMSYKMLQINA	152906	126	26
38	KEAFWDRCLSVIQLMSSKMLQINA	154117	127	27
50	KEAFWDRCLSVINLMASKMLQINA	154975	128	28
2	REAFWDRCLSVINLMSSKMLQINA	155431	128	28
25	KEAFWDRCGSVINLMSSKMLQINA	155679	128	28
56	KEAFWDRCLSVINLMSSRMLQINA	155892	129	29
49	KEAFWDRCLSVINLMYSKMLQINA	156497	129	29
67	KEAFWDRCLSVINLMSSKMLQLNA	156827	129	29
11	KESFWDRCLSVINLMSSKMLQINA	163262	135	35
37	KEAFWDRCLSVGNLMSSKMLQINA	164154	135	35
22	KEAFWDRCISVINLMSSKMLQINA	165242	136	36
19	KEAFWDKCLSVINLMSSKMLQINA	166245	137	37
8	KEIFWDRCLSVINLMSSKMLQINA	166249	137	37
63	KEAFWDRCLSVINLMSSKMGQINA	166937	138	38
74	KEAFWDRCLSVINLMSSKMLQING	167174	138	38
5	KQAFWDRCLSVINLMSSKMLQINA	167318	138	38
68	KEAFWDRCLSVINLMSSKMLQVNA	167911	139	39
10	KEMFWDRCLSVINLMSSKMLQINA	168848	139	39
58	KEAFWDRCLSVINLMSSKALQINA	170159	140	40
55	KEAFWDRCLSVINLMSMKMLQINA	170786	141	41
12	KEAYWDRCLSVINLMSSKMLQINA	171316	141	41
24	KEAFWDRCASVINLMSSKMLQINA	171581	142	42
43	KEAFWDRCLSVINAMSSKMLQINA	172298	142	42
51	KEAFWDRCLSVINLMMSKMLQINA	174387	144	44
57	KEAFWDRCLSVINLMSSKSLQINA	175515	145	45
9	KEVFWDRCLSVINLMSSKMLQINA	175785	145	45
44	KEAFWDRCLSVINGMSSKMLQINA	176026	145	45
69	KEAFWDRCLSVINLMSSKMLQANA	178232	147	47
72	KEAFWDRCLSVINLMSSKMLQIQA	181214	150	50
23	KEAFWDRCVSVINLMSSKMLQINA	181448	150	50
45	KEAFWDRCLSVINLSSSKMLQINA	182017	150	50
30	KEAFWDRCLSAINLMSSKMLQINA	184482	152	52
21	KEAFWDRMLSVINLMSSKMLQINA	185771	153	53
36	KEAFWDRCLSVANLMSSKMLQINA	186090	154	54
31	KEAFWDRCLSGINLMSSKMLQINA	186098	154	54
6	KEGFWDRCLSVINLMSSKMLQINA	186614	154	54
27	KEAFWDRCLTVINLMSSKMLQINA	189548	156	56
7	KELFWDRCLSVINLMSSKMLQINA	190811	157	57
47	KEAFWDRCLSVINLCSSKMLQINA	191850	158	58
35	KEAFWDRCLSVVNLMSSKMLQINA	192606	159	59
46	KEAFWDRCLSVINLASSKMLQINA	193507	160	60
65	KEAFWDRCLSVINLMSSKMLEINA	194286	160	60
54	KEAFWDRCLSVINLMSAKMLQINA	194650	161	61
52	KEAFWDRCLSVINLMSTKMLQINA	195577	161	61
60	KEAFWDRCLSVINLMSSKMIQINA	196418	162	62
39	KEAFWDRCLSVIELMSSKMLQINA	196444	162	62
73	KEAFWDRCLSVINLMSSKMLQIEA	197176	163	63
32	KEAFWDRCLSLINLMSSKMLQINA	197903	163	63
40	KEAFWDRCLSVIDLMSSKMLQINA	198851	164	64
71	KEAFWDRCLSVINLMSSKMLQIDA	199652	165	65
66	KEAFWDRCLSVINLMSSKMLDINA	199850	165	65
62	KEAFWDRCLSVINLMSSKMAQINA	203269	168	68
26	KEAFWDRCLAVINLMSSKMLQINA	204497	169	69
70	KEAFWDRCLSVINLMSSKMLQGNA	207194	171	71
64	KEAFWDRCLSVINLMSSKMLNINA	217093	179	79