Fig.5.

1 w-TP. Immunostaining for axons. The spinal cord was immunostained for axons. This micrograph was at a level several sections away from that of Fig. 4. A: Axons (red) are found in the remaining host spinal cord tissue and the astrocyte-devoid areas shown in Fig. 4A. CPEC clusters of various sizes (green) are located within and at the border of the lesion. B: This simple fluorescent micrograph was taken from a section adjacent to that of panel A, showing clusters of engrafted CPECs. C: Panel B was merged in the unstained transmitted-light picture to show the localization of engrafted CPECs in the spinal cord tissue. Arrows point to some of the clusters. a: Rectangle a in panel A was enlarged. Small clusters of CPECs (green) can be seen among axons that have various diameters and irregular orientations. b: Rectangle b in panel A was enlarged. Axons are scarce around and within the stacked CPECs (green). Scale: 500 μm for A-C, and 100 μm for a and b.