Fig.1.

Tau can be hydrolyzed at its C-terminus in the presence of Cycloheximide. A) Schematic representation of the antibodies used in this study. 9E10 was used to recognize myc tag which was fused into N-terminus of Tau, the epitope recognized by Tau46 is located within the range of 404–441aa in the 2N4R isoform of human tau. B) Cycloheximide chase analysis of human tau (2N4R) in Drosophila Kc cells using antibody Tau46 or 9E10. C) Quantification of the relative levels of tau after the treatment described in (B). The amount of tau was normalized to the tubulin levels. The results are the means ± S.E. from three independent experiments. D) Cycloheximide chase analysis of human tau (0N4R) in Drosophila Kc cells using antibody 9E10 and Tau46. E) Quantification of the relative levels of tau after the treatment described in (D). The amount of tau was normalized to the tubulin levels. The results are the means ± S.E. from three independent experiments.