Fig.2.

Tau can be truncated by protease during degradation and the truncation can be inhibited by caspase inhibitor. A) Western blot analysis of full-length human tau treated with alkaline phosphatase. At 48-h post transfection, Kc cells were treated with or without 100 μg/ml cycloheximide for 24 h, and the cell lysates were treated with or without alkaline phosphatase at 37°C for 1 h. B) Western blot analysis of full-length human tau in Drosophila Kc cells treated with different protease inhibitors in the presence of cycloheximide (100 μg/ml). At 48-h post transfection, Kc cells were treated with 100 μg/ml cycloheximide and protease inhibitors for 24 h. C) Alkaline phosphatase treatment of full-length human tau from cycloheximide-treated Drosophila Kc cells in the presence or absence of caspase inhibitor Z-VAD. At 48-h post transfection, Kc cells were treated with 100 μg/ml cycloheximide and Z-VAD for 24 h, then the cell lysates were treated with or without alkaline phosphatase at 37°C for 1 h.