Fig.4.

Cycloheximide induces the apoptosis of Kc cells. A) The apoptosis of Kc cells under CHX treatment. Kc cells were treated with cycloheximide (100 μg/ml) for the indicated times, TUNEL assay was used to detect apoptosis of Kc cells. PC, positive control, add 100 μL DNaseI incubated for 10 min before fixation of the Kc cells; NC, negative control, Kc cells were treated with DMSO for 48 h. B) The statistical histogram of TUNEL signal in Kc cells with different cycloheximide treatment duration (n = 3, ^**p <  0.001).