Fig.5.

Tau can be cleaved primarily at Asp421with the treatment of Cycloheximide. A) Cycloheximide chase analysis of mutant human tau [tau(S422A) and tau(S422E)] in Drosophila Kc cells using 9E10 antibody. B) Quantification of the relative levels of tau after the treatment described in (A). The amount of tau was normalized to the tubulin levels. The results are the means ± S.E. from three independent experiments. C) Cycloheximide chase analysis of mutant human tau [tau(S422A) and tau(S422E)] in Drosophila Kc cells using Tau46 antibody. Tubulin was used as the loading control in all western blot analyses. D) Quantification of the relative levels of tau after the treatment described in (C). The amount of tau was normalized to the tubulin levels. The results are the means ± S.E. from three independent experiments and data points indicated with asterisks are significant.  ^*p <  0.05.