Subject area	Biology
More specific subject area	Nanoparticles: elastic liposomes.
Type of data	Figure, table.
How data were acquired	Gel imaging system (ChemiDoc-It Imager, UVP or ChemiDoc XRS+, Bio-Rad), Cryo-electron microscopy (FEI Tecnai 20, FEI or CM120, Philips), Microplate reader (FLUOstar OPTIMA, BMG Labtech or EnVision Multilabel Plate Reader, Perkin Elmer), Flow cytometer (BD LSRFortessa, BD Biosciences), Real-time PCR detection system (MyiQ iCycler, Bio-Rad), Dynamic light scattering system (Zetasizer Nano series, Malvern).
Data format	Raw, analyzed.
Experimental factors	The RNAi encapsulation efficiency has been analyzed for several liposomal formulations. (Psoriasis-induced) keratinocytes and melanocytes were cultured in vitro prior to transfection with empty liposomes or loaded lipoplexes, to assess respectively cytotoxicity and transfection efficiency. Split thickness skin was prepared from thawed human skin, obtained from healthy volunteers, to use in permeation studies.
Experimental features	The encapsulation efficiency was evaluated using gel electrophoresis. in vitro transfection efficiency was analyzed by means of flow cytometry and Western blot. Franz diffusion cell permeation study was used to measure the skin penetration capacity. Cryo-TEM, CellTiter-Glo assay and dynamic light scattering was used to assess the morphology, cytotoxicity and particle size of the (loaded) liposomes, respectively.
Data source location	Ghent, Belgium.
Data accessibility	Data are included in this article.