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. 2016 Jun 16;2016:6586857. doi: 10.1155/2016/6586857

Figure 9.

Figure 9

Electrophoretic mobility shift assay to study DNA-binding activity. Electrophoretic mobility shift assay was performed to study the DNA-binding activity of NF-κB in the ICB of EIU rats (a) and in RAW 264.7 cells treated with LPS (b). The results showed that the DNA-binding activity increased in the LPS group, and treatment with bortezomib effectively lowered the NF-κB activity. Lane 1: p50 subunit of NF-κB. Lane 2: free probe. Lane 3: control group. Lane 4: LPS-treated group. Lane 5: LPS with high-dose bortezomib. Lane 6: LPS with low-dose bortezomib. Lane 7: competition with 100x unlabeled NF-κB probe. Lane 8: anti-p65 antibody supershift band.