Subject area |
Molecular biology |
More specific subject area |
DNA break repair |
Type of data |
Image, graph, figure |
How data was acquired |
Olympus Provis AX70 microscope, BD LSR II flow cytometer, BioTek Synergy 4 hybrid microplate reader. |
Data format |
Raw |
Experimental factors |
Cells were treated with 10 µM THD 1 h before drug washout and irradiation, whereas cell treatment with 50 µM GA followed 30 minutes after radiation and extended up to indicated experimental time points or 16 h before placement in drug-free medium.
|
Experimental features |
NS-SV-AC and stably transfected shRNA TLK1 cells were grown in complete KGM-2 medium. Cells were treated with drug before or after radiation, and cells were analyzed by colony formation assays, single cell alkaline gel electrophoreses, or Annexin V staining at indicated times. Radiation-generated reactive oxygen species was analyzed using CM-H2DCFDA. Repair of restriction enzyme (I SceI)- induced double strand break was studied in HEK293-PC222 cells. Cell population that became RFP positive was quantified by FACS analyses. |
Data source location |
Shreveport, LA |
Data accessibility |
Data accessible in the article |