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. 2016 Mar 30;7:1073–1077. doi: 10.1016/j.dib.2016.03.075
Subject area Molecular biology
More specific subject area DNA break repair
Type of data Image, graph, figure
How data was acquired Olympus Provis AX70 microscope, BD LSR II flow cytometer, BioTek Synergy 4 hybrid microplate reader.
Data format Raw
Experimental factors Cells were treated with 10 µM THD 1 h before drug washout and irradiation, whereas cell treatment with 50 µM GA followed 30 minutes after radiation and extended up to indicated experimental time points or 16 h before placement in drug-free medium.
Experimental features NS-SV-AC and stably transfected shRNA TLK1 cells were grown in complete KGM-2 medium. Cells were treated with drug before or after radiation, and cells were analyzed by colony formation assays, single cell alkaline gel electrophoreses, or Annexin V staining at indicated times. Radiation-generated reactive oxygen species was analyzed using CM-H2DCFDA. Repair of restriction enzyme (I SceI)- induced double strand break was studied in HEK293-PC222 cells. Cell population that became RFP positive was quantified by FACS analyses.
Data source location Shreveport, LA
Data accessibility Data accessible in the article