Skip to main content
. 2016 Jun 30;6:28944. doi: 10.1038/srep28944

Figure 3. Inhibition of the MeHg-mediated Akt/CREB/Bcl-2 signaling pathway by an Akt inhibitor in SH-SY5Y cells.

Figure 3

SH-SY5Y cells were pretreated with wortmannin (0.5 μM) for 30 min, and then the cells were exposed to MeHg for 12 h. The cells were also exposed to MeHg for 12 h in the presence or absence of LY294002 (5 μM). The whole cell lysates were subjected to western blotting and the band intensities were quantified (A). The cells were transfected with CRE-luciferase and then treated with wortmannin (0.5 μM) for 30 min following exposure of MeHg for 6 h. The luciferase assay was performed as described in the materials and methods (B). *p < 0.05 vs. control MeHg (0 μM). #p < 0.05 vs. control MeHg (0.5 μM or 1 μM). Each value is the mean ±  S.D. of three determinations.