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. 2016 Jun 30;6:28944. doi: 10.1038/srep28944

Figure 6. S-Mercuration of Akt and CREB by MeHg in SH-SY5Y cells.

Figure 6

SH-SY5Y cells were treated with MeHg for 3 h and a BPM-precipitation assay was performed. The precipitated proteins were detected by western blotting with the indicated antibodies (A). The cell lysate before the separation was used to estimate the alteration of protein expression by MeHg stimulation. The band intensities were quantified (B). Recombinant CREB (0.5 mg/mL) was reacted with MeHg (20 μM) for 30 min at 37 °C, and then the proteins were subjected to SDS-PAGE following Coomassie brilliant blue staining (C, upper). The CREB protein on the gel was cut out using a gel-band cutter and the total Hg concentration in the CREB was analysed by AAS. The mercury content was expressed as Hg ng/ng of protein (C, lower). *p < 0.05 vs. control. Each value is the mean ±  S.D. of three determinations.