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. 2016 Jun 30;6:29003. doi: 10.1038/srep29003

Figure 5. Adrenomedullin and ROCK1 inhibition counteract MLC phosphorylation and stress fibre formation.

Figure 5

(A) Western blot experiments for the indicated proteins show that both ADM treatment (100 nM) and ROCK1 inhibition with Y27632 (Y27, 10 μM) for 1 h reverse the increase in MLC phosphorylation after cortactin depletion (red box), while total MLC levels are unaffected. γ-tubulin served as loading control. Graph shows quantification of p-MLC from 3 independent experiments (WT = ctrl shRNA; KD = cortactin shRNA). **p < 0.01; ***p < 0.001. (B) Immunofluorescence images of MLEC showing that both ROCK1 inhibition (Y27632) and ADM treatment counteract the increase in stress fibres (red) in the absence of cortactin (KO). VE-cadherin as cell contact staining is shown in green. Representative images from three independent experiments are shown. Bar = 20 μm.