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. 2016 Jun 30;6:28218. doi: 10.1038/srep28218

Figure 2. Scarce ovarian tissues exist in follicular aspirates.

Figure 2

(A) Scheme of transvaginal oocyte retrieval performed in IVF centers showing preantral follicles and FGSCs possibly captured along with the mature oocytes. COC, cumulus-oocyte complex; OSE, ovarian surface epithelium. (B) Representative image of FAs in a 50-mL tube obtained from IVF centers. (C) A 30-μm nylon cell strainer with ovarian tissues and granulose cell aggregates from FAs (left). The red box is enlarged on the right. (D) Representative images of ovarian tissues (left, middle) and granulosa cell aggregates (right) captured from FAs showing distinct morphologies. (E) Proportion of samples containing ovarian tissues. (F) Bright-field images of human ovarian cortical tissue containing preantral follicles captured from FAs. The left image is enlarged on the right showing preantral follicles (white arrows). (G) After 2 days of culture in vitro, the preantral follicles (white arrows) were visible. Scale bars: 50 μm (D), 100 μm (F,G).