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. 2016 Jun 30;6:28832. doi: 10.1038/srep28832

Figure 3. HAECs have greater angiogenic potential than HUVECs owing to differences in collagen fiber stiffness and porosity.

Figure 3

(a) Electron microscopy images of the effect of pH on collagen fiber stiffness. Scale bar = 1 μm. (b,c) Phase contrast images of collagen fiber stiffness in the 3D microfluidic angiogenesis system and quantification of vascular density. Scale bar = 150 μm. (d,e) Phase contrast and immunofluorescence images of the vascular density of HUVECs and HAECs filled with laminin-enriched gel obtained by incubating the cells with antibody followed by F-actin (red) and DAPI (blue) staining. Scale bar = 150 μm. *p < 0.05, **p < 0.01 and ***p < 0.001 versus HUVEC group.