Table 1. Kinetic parameters, K m (μM) and V max (pmol/mg protein/min) of the uptake of fluorescent dyes.
| Mate3 | Mate4 | Mate6 | Mate7 | Mate8 | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Km | vmax | Km | vmax | Km | vmax | Km | vmax | Km | vmax | |
| DAPI | 2.83 ± 0.34 | 436 ± 21.8 | 0.29 ± 0.04 | 47.7 ± 2.27 | 0.26 ± 0.05 | 44.3 ± 1.80 | 1.44 ± 0.19 | 193 ± 9.15 | 0.55 ± 0.07 | 24.4 ± 0.70 |
| ASP+ | 13.4 ± 1.55 | 296 ± 14.0 | 4.49 ± 0.63 | 23.6 ± 1.23 | 6.23 ± 1.02 | 160 ± 10.8 | 3.51 ± 0.27 | 52.3 ± 1.26 | 2.34 ± 0.37 | 22.2 ± 1.09 |
| Rh123 | 8.52 ± 1.25 | 105 ± 5.16 | 0.32 ± 0.03 | 1.03 ± 0.03 | 1.73 ± 0.16 | 55.6 ± 1.48 | 0.22 ± 0.03 | 4.79 ± 0.17 | 0.88 ± 0.14 | 12.3 ± 0.61 |
| EtBr | 5.56 ± 0.50 | 825 ± 25.5 | 0.45 ± 0.08 | 37.4 ± 2.02 | 2.98 ± 0.18 | 504 ± 9.79 | 0.79 ± 0.19 | 26.3 ± 1.82 | 0.85 ± 0.12 | 17.0 ± 0.59 |
| Amiloride | 33.4 ± 4.57 | 53.1 ± 4.0 | NT | NT | 11.1 ± 2.12 | 24.8 ± 1.54 | 20.6 ± 4.20 | 19.8 ± 1.84 | NT | NT |
| Berberine | 14.4 ± 5.14 | 242 ± 46.9 | NT | NT | 8.35 ± 1.00 | 215 ± 9.72 | 1.76 ± 0.38 | 28.0 ± 2.39 | NT | NT |
4-(4-(dimethylamino)styryl)-N-methylpyridinium iodide (ASP+), 4′,6-diamidino-2-phenylindole (DAPI), rhodamine 123 (Rh123), ethidium bromide (EtBr), berberine and amiloride for zebrafish Mates (Mate3, 4, 6, 7 and 8). Each value represents mean ± SD from triplicate determinations of a representative experiment. NT – no transport found. Dose response graphs for each dye are shown in Fig. S3A–E.