Table 2. CNS distribution of viral antigens and virus-induced lesions in terminally-ill mice.

		Class-IA				Class-IB				Class-II
		EV-A71 antigens		Pathology		EV-A71 antigens		Pathology		EV-A71 antigens		Pathology
Cerebral cortex	Motor cortex	+++a	100%b (5/5)	+++c	80%d (4/5)	+++	80% (4/5)	+++	80% (4/5)		0% (0/5)		0% (0/5)
	Sensory cortex	++	60% (3/5)	++	80% (4/5)	++	80% (4/5)	++	80% (4/5)	+	20% (1/5)	+	20% (1/5)
Hippocampus		++	60% (3/5)	+	60% (3/5)	+	40% (2/5)	+	20% (1/5)	+	20% (1/5)	+	20% (1/5)
Diencephalon	Thalamus	+++	100% (5/5)	+++	60% (3/5)	++	60% (3/5)	++	20% (1/5)	+	20% (1/5)	+	20% (1/5)
	Hypothalamus	+++	80% (4/5)	+++	80% (4/5)	++	60% (3/5)	++	60% (3/5)	+	20% (1/5)	+	20% (1/5)
Mesencephalon		+++	100% (5/5)	+++	100% (5/5)	+	60% (3/5)	++	60% (3/5)	+	20% (1/5)	+	20% (1/5)
Metencephalon	Cerebellum	++	80% (4/5)	++	80% (4/5)	+	40% (2/5)	+	60% (3/5)		0% (0/5)		0% (0/5)
	Pons	++	80% (4/5)	++	80% (4/5)	++	20% (1/5)	++	60% (3/5)		0% (0/5)		0% (0/5)
Myelencephalon	Medulla	+++	100% (5/5)	+++	100% (5/5)	++	40% (2/5)	++	60% (3/5)	+	20% (1/5)	+	20% (1/5)
Spinal cord	Cervical	++	100% (5/5)	++	100% (5/5)	++	80% (4/5)	++	60% (3/5)		0% (0/5)		0% (0/5)
	Thoracic	++	100% (5/5)	++	100% (5/5)	++	80% (4/5)	++	80% (4/5)		0% (0/5)		0% (0/5)
	Lumbar	++	100% (5/5)	++	100% (5/5)	++	80% (4/5)	++	60% (3/5)	+	20% (1/5)	+	20% (1/5)

^aDensity of viral antigens detected per slide: +, <10 positive neurons; ++, 10–20 positive neurons; +++, >20 positive neurons.

^bPercentage of animals exhibiting viral antigens in the specified brain region (n = 5).

^cDistribution of pathologic lesions in nervous tissues: +, <5 lesions; ++, 5–10 lesions; +++, >10 lesions.

^dPercentage of animals exhibiting lesions in the specified brain region (n = 5).