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. 2016 Jun 30;6:29131. doi: 10.1038/srep29131

Figure 1. Vaccine constructs and Tat expression.

Figure 1

(A) Schematic representation of the vaccine constructs; (B) reducing Western blot analysis of Tat in cell lysates from HEK293T cells transfected with plasmid DNA encoding the different forms of Tat (tracks 2–5) and (C) non-reducing Western blot analysis of Tat in supernatant fluids of HEK293T cells transfected with pVAX-Tat-IMX313 (track 2) or pVAX-sTat-IMX313 (track 3) DNA. The 42 kDa β-actin protein was used as loading control for the Western blot. Blots in Fig. 1B,C are cropped for clarity and conciseness; full length blots of these figures are presented in supplementary figure S1A,B.