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. 2016 May 10;25(12):909–921. doi: 10.1089/scd.2015.0384

FIG. 4.

FIG. 4.

siRNA knockdown of EZH2 promotes expression of ZBTB16, MX1, and FHL1 in OB. MSC were treated with negative control siRNA, EZH2 siRNA #1, and EZH2 siRNA #2. MSC were cultured for 14 days with osteogenic differentiation media. (A) Knockdown of EZH2 by siRNA was confirmed by RT-PCR. (B) RT-PCR reveals a significant increase in RUNX2 expression compared with negative control-treated OB. (C) ZBTB16, MX1, and FHL1 gene expression was upregulated in EZH2 siRNA-treated OB compared with negative control-treated OB. Mean values ± SEM, n = 3 independent MSC donors (*P < 0.05, 1-way ANOVA with Dunnett's multiple comparison test).