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. 2016 Jun 30;6:29032. doi: 10.1038/srep29032

Figure 2. VHL loss induces morphologic and molecular changes indicative of EMT.

Figure 2

(a) Phase contrast 10X images of RC and RVN cells at low (left) and high (right) densities. (b) Growth chart of RC versus RVN cells. (c) Western blot for murine E-cadherin and β-actin in RC and RVN cells. Normalized quantification by densitometry shown above blot. Gene expression was analysed by RT-PCR for (d) E-cadherin, (e) N-cadherin, (f) α-SMA and g) MMP-9. n = 3 for proliferation assay and RT-PCR, **denotes p < 0.01, ***denotes p < 0.001.