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. 2016 Jun 30;6:29032. doi: 10.1038/srep29032

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Copyright © 2016, Macmillan Publishers Limited

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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RC and RVN cells were implanted under the kidney capsule of Nu/J mice. Animals were sacrificed at four weeks following implantation. (a) Representative bioluminescent images at day of endpoint are shown. Primary tumours (b) and lungs (c) were weighed and quantified for the two groups. (d) H&E stains are shown from primary tumour and whole lung. (e) Immunostaining for VHL in representative RC and RVN primary tumours shown at 20X. (f) 4X H&E representative images of RC and RVN lung nodules. (g) Lung nodules were counted and quantified. Gene expression of Cas9 (h) and eGFP (i) in the peripheral blood was assessed as a measure of circulating tumour cells. n = 3–4 animals per group for weights and RT-PCR, n = 6–8 per group for lung nodule counts *denotes p < 0.05.