Figure 5. Workflow of the high-throughput β-hematin assay developed by Sandlin et al.

Test compounds were delivered using a noncontact liquid handler, followed by the addition of buffer, hemin and detergent. The 384-well plates incubated at 37°C for 4 h before the addition of 5% (v/v) pyridine. The absorbance values of test compounds were compared with positive and negative controls to establish hits [74].