Study Type	Effect Studied	Experimental Model	Result	Reference
In vitro	Activity.	Susceptibility testing by the macro dilution reference method of the National Center for Clinical Laboratory Standards (NCCLS).	In vitro inhibitory activity of SPA-S-843 against Aspergillus sp was superior than that of amphotericin B. While the activity against R oryzae, P variotti, Penicillium sp and S schenkii were similar. The MFC values were 12.53, 12.00 and 19.20 µg/ml against Aspergillus sp, Penicillium sp and S schenkii, respectively.	374266
In vitro	Activity.	Broth and agar microdilution by the NCCLS.	SPK-843 had significantly lower MIC values than amphotericin B against S cerevisae, C tropicalis, C neoformans and C glabrata. SPK-843 was most fungicidal against C krusei, C tropicalis and C albicans.	308269
In vitro	Activity.	Contact test and germ tube inhibition test.	SPK-843 killed C albicans in 3 min. The best fungicidal activity occurred in a medium with low electrical conductivity (0.001 M buffer strength) and pH 5.8. The activity of SPK-843 is associated with the induction of greater cellular damage following increasing leakage of K+. SPK-843 produced 80% leakage in 4 min, while amphotericin B produced 35% in the same time.	358056
In vivo	Activity.	CD-1 mice intravenously infected with C albicans.	The activity of SPK-843 was 2-fold higher than D-AMPH at doses of 0.2 to 0.4 mg/kg/day for 5 days. At 0.8 mg/kg/day for 5 days, SPK-843 completely eradicated C albicans in eight out of ten mice, whereas the same dose of D-AMPH had no effect.	382489
In vivo	Efficacy.	CD-1 mice infected with 5 × 106 C albicans KSG-27 cells.	SPK-843 (0.25 mg/kg) reduced viable C albicans cell count in the kidney to less than 1/100 of control on day 1. At the same dose, SPK-843 eradicated fungi by day 4 from 50 to 100% of mice, whereas amphotericin B required a dose of 1 mg/kg to reach these eradication levels.	568943