. Author manuscript; available in PMC: 2016 Jun 30.

Published in final edited form as: Curr Protoc Toxicol. 2016 Feb 1;67:20.11.1–20.11.25. doi: 10.1002/0471140856.tx2011s67

Table 1.

LA-QPCR primers, targets and conditions optimized for LongAmp PCR Kit

Species	Genome	Forward Primer Seq	Reverse Primer Seq	Annealing Temperature (°C)	Cycle Number	Reference

C. elegans	mt	10.9 kb mito fragment		64	26	This unit, Hunter et al. (2010)
	mt	5’-CCA TCA ATT GCC CAA AGG GGA GT-3’	5’-TGT CCT CAA GGC TAC CAC CTT CTT CA-3’	64	26	This unit, Hunter et al. (2010)

	nuc	9.3 kb nuc fragment from the unc-2 gene		64	29	This unit, Hunter et al. (2010)
	nuc	5’-TGG CTG GAA CGA ACC GAA CCA T-3’	5’-GGC GGT TGT GGA GTG TGG GAA G-3’	64	29	This unit, Hunter et al. (2010)

M. musculus	mt	10.9 kb mito fragment		64	17	This unit, Furda et al. (2012)
	mt	5’-GCC AGC CTG ACC CAT AGC CAT AAT AT-3’	5’-GAG AGA TTT TAT GGG TGT AAT GCG G-3’	64	17	This unit, Furda et al. (2012)

	nuc	8.7 kb nuc fragment of the β-globin gene, accession number X14061		62	27	This unit, Ayala-Torres et al. (2000)
	nuc	5’-TTG AGA CTG TGA TTG GCA ATG CCT-3’	5’-CCT TTA ATG CCC ATC CCG GAC T-3’	62	27	This unit, Ayala-Torres et al. (2000)

H. sapiens	mt	8.9 kb mito fragment, accession number J01415		64	17	This unit, Furda et al. (2012)
	mt	5’-TCT AAG CCT CCT TAT TCG AGC CGA-3’	5’-TTT CAT CAT GCG GAG ATG TTG GAT GG-3’	64	17	This unit, Furda et al. (2012)

	nuc	13.5 kb nuc fragment from the 5’ flanking region near the β-globin gene, accession number J00179		64	28	This unit, Furda et al. (2012)
	nuc	5’-CGA GTA AGA GAC CAT TGT GGC AG-3’	5’-GCA CTG GCT TAG GAG TTG GAC T-3’	64	28	This unit, Furda et al. (2012)

D. rerio	mt	10.3 kb mito fragment		66	29	This unit, Hunter et al. (2010)
	mt	5’-TTA AAG CCC CGA ATC CAG GTG AGC-3’	5’-GAG ATG TTC TCG GGT GTG GGA TGG-3’	66	29

	nuc	10.7 kb nuc fragment of the AHR2 gene		68	30
	nuc	5’-AGA GCG CGA TTG CTG GAT TCA C-3’	5’-GTC CTT GCA GGT TGG CAA ATG G-3’	68	30

F. heteroclitus	mt	13.1 kb mitochondria fragment, accession number NC_012312		63	22	This unit, Hunter et al. (2010), Jung et al. (2009)
	mt	5’-AAG GAA ACA AGG AGC CGG TA-3’	5’-ACG TAG CGA GAA GGG TTA GG-3’	63	22

	nuc	11.5 kb fragment of the CFTR gene, accession number AY028263		65	29
	nuc	5’-CAG CCG CCC GCA AAT TCT CA-3’	5’-CAG AAT GCG GGC CTT GCT GA-3’	65	29

R. norvegicus	mt	12.1 kb mito fragment		66	19	This unit
	mt	5’- TCG CCC CAA CCC TCT CCC TT -3’	5’- TCG CCC CAA CCC TCT CCC TT -3’	66	19	This unit

	nuc	12.9 kb nuc fragment from chromosome 12 genomic scaffold 5380		66	23	This unit
	nuc	5’- CCT GCT GGG CTT GCC TTG GT -3’	5’- AGC AGG GGA GGT GGA TGG GA -3’	66	23	This unit