Table 3.

Real-time PCR primers and targets

Species	Genome	Forward Primer Seq	Reverse Primer Seq	Annealing Temperature (°C)	Reference
C. elegans	mt	75 bp mito fragment of nd1 mito		60	Bratic et al. (2009)
		5’-AGC GTC ATT TAT TGG GAA GAA GAC-3’	5’- AAG CTT GTG CTA ATC CCA TAA ATG T -3’
	nuc	164 bp nuc fragment of cox4		60	Rooney et al. (2015)
		5’-GCC GAC TGG AAG AAC TTG TC-3’	5’-GCG GAG ATC ACC TTC CAG TA-3’
D. rerio	mt	195 bp nd1 mito fragment		60	This unit
		5’-CGT TTA CCC CAG ATG CAC CT-3’	5’-GTG CGA TTG GTA GGG CGA TA-3’
	nuc	90 bp nuc fragment of vtg2		60
		5’-TGG ATA CCT GAC CGA GAG CT-3’	5’-AGA CAA CTC TTA CGG CTG GC-3’
H. sapiens	mt	107 bp mito fragment of tRNA-Leu(UUR) gene		62	Venegas and Halberg (2012)
		5’-CAC CCA AGA ACA GGG TTT GT-3’	5’-TGG CCA TGG GTA TGT TGT TA-3’
	nuc	86 bp nuc fragment of β2-microglobulin gene		62
		5’- TGC TGT CTC CAT GTT TGA TGT ATC T -3’	5’- TCT CTG CTC CCC ACC TCT AAG T -3’
R. norvegicus	mt	181 bp mito fragment		60	This unit
		5’- CAA ACC TTT CCT GCA CCT CC -3’	5’- AGG CGT TCT GAT GAT GGG AA -3’
	nuc	144 bp nuc fragment from 3',5'-cyclic AMP phosphodiesterase (PDE4-1, PDE4-2) gene		62
		5’-GTT CCC GCC TTC TTC CTC TG-3’	5’-GTT TGC TTG CCG ACT CCT TG-3’
F. heteroclitus	mt	131 bp mito fragment of 16S rRNA gene		60	This unit
		5’-AAA ATT AAC GGC CCC AAC CC-3’	5’-CCG AGT TCC TTC TTC CCC TT-3’
	nuc	234 bp nuc fragment of CFTR gene		60	Hunter et al. (2010) Jung et al. (2009)
		5’-GCC GCT GCC TTC ATT GCT GT-3’	5’-ATG AGC TGG GTG TGC GCT GA-3’