Table 4.

Cloning PCR primers and conditions

Species	Genome	Target gene	Forward primer Seq	Reverse primer Seq	Amplicon (bp)	Annealing temperature (oC)	Reference
C. elegans	mt	nd-1	Not Reported	Not Reported	NR	NR	Bratic et al. (2009)
	nuc	cox-4	5’- ATCT AAGCTT GCC GAC TGG AAG AAC TTG TC -3’	5’- ATAG CTCGAG GCG GAG ATC ACC TTC CAG TA -3’	184	60	This unit
H. sapiens	mt	tRNA- Leu(UUR)	5’- ATCTAAGCTT GCC TTC CCC CGT AAA TGA TA -3’	5’- ATAG CTCGAG AGG AAT GCC ATT GCG ATT AG -3’	215	55	This unit
	nuc	B2M	5’- ATCT AAGCTT TGC TGT CTC CAT GTT TGA TGT ATC T -3’	5’- ATAG CTCGAG TCT CTG CTC CCC ACC TCT AAG T -3’	106	62	This unit
F. heteroclitus	mt	16S rRNA	5’- ATAG AAGCTT AAA ATT AAC GGC CCC AAC CC -3’	5’- ACTT CTCGAG CCG AGT TCC TTC TTC CCC TT -3’	131	64	This unit
	nuc	CFTR	5’- ATAG AAGCTT GCC GCT GCC TTC ATT GCT GT -3’	5’- AATT CTCGAG ATG AGC TGG GTG TGC GCT GA -3’	234	65	Hunter et al. (2010)
D. rerio	mt	nd-1	5’- ATCT AAGCTT CGT TTA CCC CAG ATG CAC CT -3’	5’- ATAG CTCGAG GTG CGA TTG GTA GGG CGA TA -3’	215	60	This unit
	nuc	vtg2	5’- ATCT AAGCTT TGG ATA CCT GAC CGA GAG CT -3’	5’- ATAG CTCGAG AGA CAA CTC TTA CGG CTG GC -3’	110	60	This unit

Underlined regions denote restriction sites.