Table 1.
Small RNA extraction kit comparison
| Kit | Horse ID | Serum volume (μl) | RNA concentration, Nanodrop (ng/μl) | Elution volume (μl) | Small RNA concentration, Bioanalyzer (pg/μl) | miRNA in small RNA (%) | miRNA/100 μl serum (ng) |
|---|---|---|---|---|---|---|---|
| ZR WB | 1 | 200 | 1.85 | 8 | 89.4 | 46 | 0.17 |
| ZR WB | 3 | 200 | 3.65 | 8 | 69.5 | 63 | 0.18 |
| Direct-zol | 1 | 100 | 2.70 | 50 | 15.0 | 86 | 0.64 |
| Direct-zol | 3 | 100 | 0.45 | 50 | 12.8 | 53 | 0.34 |
| miRNeasy | 1 | 200 | 56.23 | 12 | 30.4 | 72 | 0.13 |
| miRNeasy | 3 | 200 | 11.69 | 12 | 126.2 | 77 | 0.58 |
| TRIzol | 1 | 400 | 52.98 | 10 | 138.5 | 63 | 0.22 |
| TRIzol | 3 | 400 | 138.21 | 10 | 127.9 | 43 | 0.14 |
RNA extraction results with different kits used. Due to differences in serum and elution volumes used, the RNA/miRNA concentration is given in RNA per 100μl of serum. RNA was measured with Nanodrop and miRNA with Bioanalyzer. The concentration was not measurable with the Qubit high sensitivity RNA kit. Due to very low concentration, the Nanodrop results should be treated with caution