Table 3.
BAL fluid counts in mice 1, 3 and 28 days post exposure to 54 μg, 162 μg and 486 μg sanding dust from epoxy and 162 μg CB
| Control | EPOXY-REF | EPOXY-CNT | EPOCYL | CBa | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 day | 0 μg | 54 μg | 162 μg | 486 μg | 54 μg | 162 μg | 486 μg | 54 μg | 162 μg | 486 μg | 162 μg | |
| Neutrophils (x103) | 9.4 ± 2.5 | 37.8 ± 5.0** | 110.1 ± 20.8*** | 189.3 ± 16.2*** | 72.7 ± 8.8*** | 154.4 ± 20.1*** | 182.3 ± 22.2*** | 42.0 ± 7.8** | 149.7 ± 12.1*** | 188.3 ± 15.2*** | 143.7 ± 26.1*** | |
| Macrophages (x103) | 62.8 ± 5.9 | 93.5 ± 14.7 | 72.3 ± 16.7 | 49.3 ± 6.8 | 62.5 ± 5.1 | 70.6 ± 13.1 | 53.4 ± 2.9 | 85.8 ± 9.5 | 65.3 ± 11.6 | 41.4 ± 7.0 | 18.4 ± 4.5** | |
| Eosinophils (x103) | 1.0 ± 0.5 | 19.4 ± 3.7*** | 51.3 ± 8.7*** | 28.1 ± 5.0*** | 48.4 ± 9.4*** | 91.5 ± 13.7*** | 17.4 ± 8.5* | 17.6 ± 5.3** | 58.8 ± 7.9*** | 16.0 ± 7.0** | 20.2 ± 5.7** | |
| Lymphocytes (x103) | 0.8 ± 0.2 | 2.8 ± 1.0 | 3.6 ± 1.0 | 4.6 ± 1.2* | 5.2 ± 1.9 | 6.6 ± 2.7 | 6.8 ± 1.3* | 2.1 ± 0.6 | 3.7 ± 1.3 | 3.5 ± 1.2 | 0.9 ± 0.5 | |
| Epithelial (x103) | 8.8 ± 1.6 | 9.0 ± 1.7 | 10.7 ± 3.5 | 13.7 ± 4.2 | 9.7 ± 2.5 | 10.8 ± 3.1 | 13.1 ± 2.8 | 9.7 ± 2.2 | 10.6 ± 2.7 | 13.4 ± 1.7 | 9.8 ± 2.1 | |
| Total BAL cells (x103) | 82.8 ± 8.3 | 162.4 ± 21.0** | 248.0 ± 38.2*** | 285.0 ± 28.4*** | 198.5 ± 11.1*** | 334.0 ± 29.1*** | 273.0 ± 32.7*** | 157.2 ± 15.4** | 288.0 ± 22.2*** | 262.5 ± 19.9*** | 193 ± 26.4** | |
| 3 days | ||||||||||||
| Neutrophils (x103) | 2.6 ± 1.2 | 5.0 ± 1.3 | 40.9 ± 7.3*** | 94.5 ± 19.7*** | 28.0 ± 8.4*** | 26.2 ± 4.8*** | 113.5 ± 27.3*** | 10.5 ± 1.3** | 50.6 ± 8.6*** | 151.5 ± 17.6*** | 120.2 ± 24.4*** | |
| Macrophages (x103) | 57.8 ± 5.8 | 81.0 ± 12.5 | 96.9 ± 10.6 | 111.2 ± 22.2 | 78.0 ± 13.2 | 95.9 ± 16.4 | 125.1 ± 23.5* | 84.8 ± 8.6 | 146.9 ± 11.8*** | 98.6 ± 32.4 | 65.5 ± 16.3 | |
| Eosinophils (x103) | 5.3 ± 4.4 | 36.8 ± 5.8*** | 99.6 ± 22.8*** | 30.3 ± 12.9* | 95.4 ± 14.0*** | 139.5 ± 36.1*** | 32.8 ± 9.0* | 56.5 ± 22.1*** | 173.0 ± 47.7*** | 41.8 ± 9.5*** | 43.8 ± 16.3 | |
| Lymphocytes (x103) | 1.7 ± 0.6 | 2.8 ± 0.6 | 29.6 ± 7.1*** | 53.7 ± 24.2*** | 10.6 ± 2.9* | 34.2 ± 5.7*** | 35.5 ± 21.9 | 9.3 ± 3.5** | 25.5 ± 5.4*** | 14.0 ± 3.5*** | 3.1 ± 1.4 | |
| Epithelial (x103) | 7.1 ± 1.5 | 7.0 ± 0.7 | 9.5 ± 1.7 | 21.9 ± 2.4** | 8.5 ± 2.6 | 10.3 ± 3.0 | 14.1 ± 3.4 | 11.9 ± 2.5 | 14.4 ± 2.6 | 24.6 ± 2.8** | 12.8 ± 4.5 | |
| Total BAL cells (x103) | 74.5 ± 8.8 | 132.5 ± 17.2 | 276.5 ± 34.3*** | 311.5 ± 64.4*** | 220.5 ± 33.4*** | 306.0 ± 46.1*** | 321.0 ± 67.9*** | 173.0 ± 30.8*** | 410.5 ± 52.0*** | 330.5 ± 31.6*** | 254.4 ± 28.3*** | |
| 28 days | ||||||||||||
| Neutrophils (x103) | 6.8 ± 4.2 | 3.4 ± 1.4 | 8.8 ± 3.2 | 29.6 ± 5.4** | 6.9 ± 1.7 | 18.2 ± 4.1* | 30.2 ± 5.0*** | 5.3 ± 2.1 | 12.0 ± 2.4 | 53.8 ± 9.8*** | 59.3 ± 8.6*** | |
| Macrophages (x103) | 60.3 ± 5.7 | 53.1 ± 11.1 | 74.6 ± 8.4 | 100.2 ± 18.3 | 67.9 ± 12.7 | 93.1 ± 11.2 | 99.3 ± 11.9 | 50.9 ± 5.5 | 75.0 ± 5.9 | 84.2 ± 10.1 | 118.2 ± 29.5 | |
| Eosinophils (x103) | 20.4 ± 10.1 | 18.5 ± 8.1 | 3.2 ± 0.7 | 3.2 ± 0.8 | 8.8 ± 2.7 | 6.3 ± 3.4 | 2.2 ± 1.0 | 10.0 ± 3.6 | 2.2 ± 0.9 | 2.5 ± 0.7 | 1.7 ± 1.0 | |
| Lymphocytes (x103) | 4.9 ± 1.6 | 6.2 ± 2.1 | 5.7 ± 1.3 | 7.9 ± 1.6 | 4.7 ± 1.4 | 6.3 ± 0.8 | 10.1 ± 3.1 | 10.7 ± 4.0 | 6.4 ± 1.1 | 8.6 ± 1.3 | 36.4 ± 12.6 | |
| Epithelial (x103) | 9.8 ± 1.9 | 11.8 ± 3.5 | 7.2 ± 2.6 | 13.5 ± 2.2 | 7.8 ± 2.6 | 7.6 ± 1.8 | 14.6 ± 2.7 | 10.1 ± 3.9 | 11.0 ± 2.1 | 12.9 ± 2.8 | 18.0 ± 3.0 | |
| Total BAL cells (x103) | 102.3 ± 16.3 | 93.0 ± 19.2 | 99.5 ± 11.8 | 154.5 ± 18.1** | 96.0 ± 17.0 | 131.5 ± 15.5 | 156.5 ± 12.8 | 87.0 ± 10.4 | 106.5 ± 7.5 | 162.0 ± 16.5* | 233.5 ± 23.7** | |
Mean ± SEM
*p < 0.05 compared to controls, **p < 0.01 compared to controls, ***p < 0.001 compared to controls
There were no statistically significant differences between the three sanding dusts at the 0.05 level
aOne mouse in the 162 μg CB group on day 3 post-exposure was considered an outlier because the total number of BAL cells was 10 times higher than the rest of the group. Therefore the BAL results from this mouse were excluded from the BAL dataset