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. 2016 Jun 29;17:96. doi: 10.1186/s12863-016-0403-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Characterization of the PdRanBP protein. a Nucleotide and deduced amino acid sequence of the coding region (cDNA) of PdRanBP from Populus deltoides. The highlighted yellow sequence indicates the conserved RAN subfamily domain. The 2Fe-2S ferredoxin-type iron-sulphur-binding domains are boxed. The EGF-like domain is underlined with a thin black line. The double-underlined region is the VWFC domain. The PdRanBP protein sequence was analysed and characterized using the SMART analysis service (http://smart.embl-heidelberg.de/). b A neighbor- joining phylogenetic tree was constructed based on an alignment of the protein sequences of AtRanBP [GenBank: AY116939.1], CaRanBP [GenBank: AJ299064.1], CcRanBP [GenBank: AB372270.1], CmRanBP [GenBank: AB015288.1], DlRanBP [GenBank: JF461291.1], FaRanBP [GenBank: FJ610236.1], GmRanBP [GenBank: AK243772.1], HaRanBP [GenBank: AF495716.1], LeRanBP [GenBank: L28714.1], LjRanBP [GenBank: Z73959.1], NtRanBP [GenBank: L16787.1], NsRanBP [GenBank: AY563049.1], OsRanBP [GenBank: AB015288.1], PsRanBP [GenBank: EF194277.1], PtRanBP6 [GenBank: XM_002308612.1], PtRanBP18 [GenBank: XM_002324810.2], RcRanBP [GenBank: XM_002515509.1], SlRanBP [GenBank: NM_001247091.1], StRanBP [GenBank: DQ222522.1], VfRanBP [GenBank: Z24678.1], VvRanBP [GenBank: FQ396597.1], hpPdRanBP [KU841447] and PdRanBP [KU841446]. MEGA 4.0 software was used for this alignment. The 23 RanBP amino acid sequences are shown in Additional file 2. The scale bar indicates the distance calculated using the multiple alignment