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. 2016 Jun 29;17:96. doi: 10.1186/s12863-016-0403-4

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — qRT-PCR analysis of secondary cell wall-associated gene expression in PdRanBP-OE, PdRanBP-DR and WT seedlings. Total RNA was isolated from three biological replicates of the wild-type (WT), PdRanBP-OE and PdRanBP-DR plants. The expression of trans-cinnamate 4-hydroxylase 1 (PtrC4H1), cinnamyl alcohol dehydrogenase 10 (PtrCAD10), caffeoyl CoA 3-O-methyltransferase 1 (PtrCCoAOMT1), glycosyltransferase 8 (PtrGT8), cinnamoyl coenzyme A reductase 7 (PtrCCR7), sucrose synthase 1 (PtrSuS1), beta-tubulin 7 (PtrTUB7), fragile fibre 1 (PtrFRA1), and two myeloblastosis (MYB) genes (PtrMYB90, PtrMYB18) were examined. TUA1 (a) and UBQ1 (b) were used as reference genes, and the expression level of each gene in the WT background was set to 1. The data are the mean ± standard errors (SE). The asterisks indicate significant differences from WT (*P < 0.05)