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. Author manuscript; available in PMC: 2017 Jun 8.
Published in final edited form as: Photochem Photobiol Sci. 2016 May 10;15(6):822–831. doi: 10.1039/c6pp00058d

Fig 1. Activated T cells incorporate higher levels of monomeric Pc 4 than resting T cells.

Fig 1

Activated or resting T cells were incubated with Pc 4 (0–300 nM) in complete medium for 2 h. To measure monomeric Pc 4 levels, flow cytometric analysis was performed using a LSR II Flow Cytometer to obtain the mean fluorescence intensity (MFI) for each sample. Pc 4 was excited by a broadband UV laser (335–365 nm) and fluorescence emission was collected with a 650-nm long-pass filter. Autofluorescence was subtracted from each sample. (A & B) Representative examples of the resting (A) and activated (B) populations are shown from 8 independent experiments (individuals). (C) Quantitative data demonstrating the average Pc 4 MFI ± SEM in resting and activated T cells.