Table 2. IucA Particle Size and Mass by SAXSa.
| RG by P(r) (Å) | DMAX by P(r) (Å) | M.W. by I0 (kDa) | M.W. by PV (kDa) | M.W. by VC (kDa) | |
|---|---|---|---|---|---|
| [IucA] (mg/mL) | |||||
| 5.30 | 41.0 | 128 | 235 | 236 | 238 |
| 3.98 | 41.4 | 131 | 231 | 239 | 240 |
| 2.65 | 41.7 | 131 | 240 | 243 | 241 |
| 1.33 | 41.7 | 131 | 256 | 248 | 241 |
| mean ± SD | 41.5 ± 0.4 | 130 ± 2 | 240 ± 11 | 241 ± 5 | 240 ± 1 |
| 0b | 41.9 | 134 | 240 | 244 | 242 |
| [IucA] w/ATP (mg/mL) | |||||
| 5.05 | 40.8 | 130 | 235 | 220 | 233 |
| 3.79 | 41.0 | 129 | 234 | 223 | 234 |
| 2.53 | 41.3 | 132 | 241 | 228 | 235 |
| 1.26 | 41.5 | 130 | 231 | 232 | 235 |
| mean ± SD | 41.2 ± 0.3 | 130 ± 1 | 235 ± 4 | 226 ± 5 | 234 ± 1 |
| 0b | 41.6 | 133 | 242 | 232 | 238 |
a
RG = radius of gyration, DMAX = maximum particle dimension, P(r) = pair distance distribution, I0 = zero-angle scattering intensity, PV = Porod volume, and VC = volume of correlation.
b
To correct for any minor concentration dependence, particle size and mass values were also calculated for scattering data extrapolated to infinite dilution.