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Antibody mix II in PBS
| Label | Marker | Clone | Dilution |
|---|---|---|---|
| FITC | CD3 | 145-2C11 | 1/300 |
| FITC | CD19 | 1D3 | 1/500 |
| PE-eFluor610 | CD11c | N418 | 1/300 |
| PE-Cy5 | CD8a | 53-6.7 | 1/600 |
| PE-Cy7 | CD11b | M1/70 | 1/800 |
| AF-647 | CD64 | X54-5/7.1 | 1/100 |
| APC-Cy7 | MHCII | M5/114.15.2 | 1/1,000 |
| eFluor 506 | Viability | 1/200 | |
| – | CD16/CD32 | 2.4G2 | 1/200 |
Note: Use of PBS is necessary for optimal staining of dead cells with the Fixable viability dye eFluor 506.
