Table 3. Mean OD450 readings for chicken (a, b, c), mouse (d) and rabbit (e) antibodies reactivity to the M2e peptide.
| Antibody | OD450 on Peptide | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| M2e 2–18 | M2e11-24 | M2e8-21 | M2e5-18 | M2e16-24 | M2e14-23 | M2e12-21 | M2e10-19 | M2e8-17 | M2e6-15 | M2e4-13 | M2e2-11 | |||||||||||||
| 2A17a | 2.11 | ✓✓✓ | 0.02 | - | 0.92 | ✓✓ | 1.66 | ✓✓✓ | 0.04 | - | 0.05 | - | 0.04 | - | 0.81 | ✓✓ | 0.87 | ✓✓ | 0.17 | - | 0.03 | - | 0.06 | - |
| 2B2a | 2.07 | ✓✓✓ | 0.01 | - | 0.58 | ✓✓ | 1.51 | ✓✓✓ | 0.02 | - | 0.04 | - | 0.05 | - | 0.26 | ✓ | 0.35 | ✓ | 0.20 | - | -0.01 | - | -0.01 | - |
| 2B47a | 1.85 | ✓✓✓ | -0.22 | - | 1.20 | ✓✓✓ | 1.32 | ✓✓✓ | -0.08 | - | -0.27 | - | -0.12 | - | -0.20 | - | 1.13 | ✓✓✓ | -0.11 | - | -0.21 | - | -0.13 | - |
| 2D10a | 2.33 | ✓✓✓ | 0.04 | - | 2.14 | ✓✓✓ | 2.14 | ✓✓✓ | 0.10 | - | 0.10 | - | 0.14 | - | 2.02 | ✓✓✓ | 2.24 | ✓✓✓ | 0.17 | - | 0.09 | - | 0.05 | - |
| PL64b | 2.29 | ✓✓✓ | 0.04 | - | 0.01 | - | 0.76 | ✓✓ | 0.08 | - | 0.08 | - | 0.14 | - | 0.02 | - | 0.10 | - | 0.10 | - | 0.07 | - | 0.10 | - |
| PL80b | 2.34 | ✓✓✓ | 0.10 | - | -0.05 | - | 1.13 | ✓✓✓ | 0.16 | - | 0.02 | - | 0.07 | - | -0.02 | - | 0.08 | - | 0.13 | - | 0.01 | - | 0.04 | - |
| Reference H5N1c | 2.02 | ✓✓✓ | -0.05 | - | -0.01 | - | -0.03 | - | 0.03 | - | 0.03 | - | 0.00 | - | 0.00 | - | 0.02 | - | 0.01 | - | 0.01 | - | 0.04 | - |
| 1N5d | 2.63 | ✓✓✓ | 0.01 | - | 0.02 | - | 0.01 | - | 0.00 | - | 0.01 | - | 0.02 | - | 0.03 | - | 0.03 | - | 0.01 | - | 0.00 | - | 0.00 | - |
| 2D16d | 3.30 | ✓✓✓ | 0.02 | - | 0.01 | - | 0.01 | - | 0.00 | - | 0.02 | - | 0.03 | - | 0.01 | - | 0.03 | - | 0.00 | - | -0.01 | - | -0.01 | - |
| 2E14d | 2.62 | ✓✓✓ | 0.35 | ✓ | 0.13 | - | 0.02 | - | 0.00 | - | 0.00 | - | 0.10 | - | 0.54 | ✓✓ | 0.01 | - | 0.01 | - | 0.00 | - | 0.00 | - |
| 2G14d | 2.26 | ✓✓✓ | 0.01 | - | 0.00 | - | 0.05 | - | 0.00 | - | 0.00 | - | 0.00 | - | 0.01 | - | 0.01 | - | 0.03 | - | 0.00 | - | 0.03 | - |
| 3D23d | 1.69 | ✓✓✓ | 0.04 | - | 0.02 | - | 0.09 | - | 0.02 | - | 0.02 | - | 0.03 | - | 0.02 | - | 0.08 | - | 0.06 | - | 0.01 | - | 0.01 | - |
| 3H4d | 2.58 | ✓✓✓ | 0.01 | - | 0.01 | - | 0.02 | - | 0.00 | - | 0.01 | - | 0.02 | - | 0.01 | - | 0.02 | - | 0.03 | - | 0.00 | - | -0.01 | - |
| Rab-1e | 1.86 | ✓✓✓ | -0.10 | - | 0.08 | - | 1.82 | ✓✓✓ | -0.13 | - | -0.19 | - | -0.14 | - | -0.13 | - | 0.13 | - | 1.46 | ✓✓✓ | 1.31 | ✓✓✓ | -0.04 | - |
| Rab-2e | 0.49 | ✓ | -0.48 | - | -0.34 | - | 0.38 | ✓ | -0.53 | - | -0.53 | - | -0.52 | - | -0.52 | - | -0.45 | - | 0.42 | ✓ | 0.24 | ✓ | -0.40 | - |
| Rab-3e | 1.64 | ✓✓✓ | -0.16 | - | 0.01 | - | 1.46 | ✓✓✓ | -0.25 | - | -0.25 | - | -0.23 | - | -0.25 | - | -0.04 | - | 1.27 | ✓✓✓ | 1.13 | ✓✓✓ | -0.10 | - |
| Rab-4e | 0.68 | ✓✓ | -0.43 | - | -0.28 | - | 0.55 | ✓✓ | -0.46 | - | -0.46 | - | -0.45 | - | -0.45 | - | -0.35 | - | 0.60 | ✓✓ | 0.39 | ✓ | -0.34 | - |
| Rab-5e | 1.26 | ✓✓✓ | -0.20 | - | -0.04 | - | 1.45 | ✓✓✓ | -0.22 | - | -0.22 | - | -0.20 | - | -0.20 | - | -0.02 | - | 1.14 | ✓✓✓ | 1.01 | ✓✓✓ | -0.14 | - |
| Rab-6e | 1.41 | ✓✓✓ | -0.26 | - | -0.01 | - | 1.76 | ✓✓✓ | -0.30 | - | -0.30 | - | -0.28 | - | -0.28 | - | -0.13 | - | 0.94 | ✓✓ | 1.44 | ✓✓✓ | -0.12 | - |
| Rab-7e | 1.46 | ✓✓✓ | -0.27 | - | -0.03 | - | 1.25 | ✓✓✓ | -0.31 | - | -0.30 | - | -0.29 | - | -0.29 | - | -0.14 | - | 1.34 | ✓✓✓ | 1.01 | ✓✓✓ | -0.13 | - |
| Rab-8e | 0.68 | ✓✓ | -0.43 | - | -0.28 | - | 0.55 | ✓✓ | -0.46 | - | -0.46 | - | -0.45 | - | -0.45 | - | -0.35 | - | 0.60 | ✓✓ | 0.39 | ✓ | -0.34 | - |
For statistical analysis, please refer to S1 File.
aChickens exposed to A/Ck/West Java/Sbg-29/2007
bChickens exposed to A/Ck/West Java/Sbg-29/2007
cChickens exposed to A/chick/Scotland/59
dMice immunised with KLH-M2e2-19
eRabbits immunised with KLH-M2e2-19