Table 4. Summary of epitopes recognized on influenza A virus M2e protein by different antibodies.
| Antibody type and designation | Antibody source | Immunogen | Epitope sequence (Identifying Antibody) | Residue length | References |
|---|---|---|---|---|---|
| Polyclonal (AS1, AS2, AS3, AS4) |
Rabbit | Fusion-M2e (BSA) | 2SLLTEVETPIR12 | 11 | [13] |
| Monoclonal (8C6, 1B12) |
Mice | Fusion-M2e (GST) |
6EVETPIRN13 2SLLTEVETPIRNEW15 |
8 14 |
[39, 44, 45] |
| Monoclonal | Mice | Live virus & synthetic peptide | 4LTEVETPIRNEWG16 | 13 | [43] |
| Monoclonal (L66, N547, Z3G1, C40G1, 14C2) |
Human (λ HAC or KM™ mice) |
Fusion-M2e (BSA) |
2SLLTEVETPIRNEWG16 (L66) 3LLTEVETPIRNEWG16 (N547) 3LLTEVETPIR12 (Z3G1)9TPIRNE14 (C40G1) 6EVETPIRNEW15 (14C2) |
15 14 10 6 10 |
[14, 42] |
| Monoclonal | Mice | Fusion-M2e (BSA) |
2SLLTEVET9 (M2e8-7) 3LLTEVETPIR12 (Z3G1) |
8 10 |
[34] |
| Monoclonal | Mice | Fusion-M2e (BSA) |
4LTEVETPIRN12 (L18) 2SLLTEVET9 (O19) 2SLLTEVETPIRNEWGCRNDSSD24 (P6) 7VETPIRN13 (S1) |
108 23 7 |
[41] |
| Polyclonal | Mice | 2SLLTEVETPIRNEWG16 | 15 | [20] | |
| Monoclonal | Human | 2SLLTE6 (TCN-031, TCN-032) | 5 | [35] | |
| Mice | Fusion-M2e (KLH) | 2SLLTEVETP10 | 9 | [40] | |
| Polyclonal & monoclonal | Chicken, mice, rabbit | Live virus & fusion-M2e (KLH) |
5TEVETPTRNEWECK18 (cAbs) 10PTRNEWEC17 (cAbs) 2SLLTEVETPTRNEWECK18 (cAbs, mAbs, rAbs)11TRNEWECK18 (mAb) 6EVETPTRN13 (rAbs) |
14 8 17 8 8 |
This study |
Difference at residue I11T between the current and previous studies corresponded to the human and swine specific M2e sequence in the former (I11) and avian specific M2e sequence in the latter (T11) [57].