Table 1. Primer sequences used for RT-qPCR.
Gene | Forward primer sequence | Reverse primer sequence |
---|---|---|
AG1 | 5’-GGAACCCTGGATGAGCATGA-3’ | 5’-AAAGGCGCTCCGATAATCTCT-3’ |
SOCS3 | 5’-AAAAATCCAGCCCCAATGGT -3’ | 5’-GGCCTGAGGAAGAAGCCTATC -3’ |
IL-4Rα | 5’-TTCCAGAACCCTGTTCCTAACC-3’ | 5’-CTCCATGTCCAGTCCGAAGGT-3’ |
HMGB1 | 5’-CCCAAAAGCGTGAGCTTAAAA-3’ | 5’-ATGATAGCCTTCGCTGGGACTA-3’ |
TLR4 | 5’- CATTTACAGTTCGTCATGCTTTCTC-3’ | 5’- AGGCATCATCCTGGCATTTT-3’ |
TLR2 | 5’- CCCTGGAGGTGTTGGATGTTAG-3’ | 5’- AGCCGAGGCAAAAACAAAGA-3’ |
HPRT | 5´-CTCATGGACTGATTATGGACAGGAC-3´ | 5´-CAGGTCAGCAAAGAACTTATAGCC-3´ |
Primer sequences for RT-qPCR reactions.