Table 1. Identification of proteins extracted from spore coat fraction of B. altitudinis SYBC hb4.
| BandNo.a | Identified protein | Accession No. | Mass | %Sequencecoverage | Mascotscore b | Peptide (s) |
|---|---|---|---|---|---|---|
| 1 | Endopeptidase | WP_012011662.1 | 86,455 | 2 | 88 | R.VLGPGGSGTTENVLAGIDR.A |
| 2 | Peptide ABC | WP_007496735.1 | 66,300 | 8 | 240 | K.VAEVIQSNFEK.V |
| transporter ATP | K.FHDGTDFNAEAVK.F | |||||
| binding protein | K.SGDSFRENPVGTGPFK.F | |||||
| 3 | Oligopeptide | WP_007499033.1 | 61,986 | 6 | 191 | K.VREDIAVVVQQQLK.E |
| binding protein | K.HILGDVPIKDLGENEFNR.K | |||||
| 4 | CotA | WP_012009087.1 | 58,882 | 6 | 117 | R.DFEATGPFFER.E |
| K.VWPYLEVEPR.K | ||||||
| 5 | Spore peptidoglycan | WP_007496056.1 | 50,407 | 8 | 96 | K.AVSPQQAIAIAR.E |
| hydrolase | R.ENNVSILYDETAQAPYFR.Y | |||||
| 6 | Hypothetical protein | WP_012011041.1 | 49,057 | 3 | 76 | R.LSQGEFLFTFDAR.D |
| 7 | Sporulation related | WP_012010687.1 | 31,115 | 21 | 254 | R.ANLNAESQGR.L |
| manganese catalase | R.EGDVVVPTTFPR.S | |||||
| (YjqC) | K.QQVSYDLFNFSR.G | |||||
| K.ELEEREGDVVVPTTFPR.S | ||||||
| 8 | Spore coat-associated | WP_007501219.1 | 29,157 | 7 | 97 | K.ENSGAINLANIKPGDR.I |
| protein | ||||||
| 9 | Transglutaminase | WP_012011070.1 | 28,507 | 10 | 99 | K.SGASFAIFQR.S |
| R.QASDLLFEVTLR.S | ||||||
| 10 | SOD | WP_007501264.1 | 22,397 | 24 | 233 | K.HHNTYVTNLNK.A |
| R.FGSGWAWLVVNNGK.L | ||||||
| K.LEITSTPNQDSPLTEGK.T | ||||||
| 11 | Purified CotA | WP_012009087.1 | 58,882 | 10 | 138 | R.DFEATGPFFER.E |
| K.VWPYLEVEPR.K | ||||||
| R.TLTLTGTQDK.Y | ||||||
| R.LGSVEVWSIVNPTR.G | ||||||
| 12 | Purified YjqC | WP_012010687.1 | 31,115 | 33 | 427 | K.ELQYEAKPSKPDPLYAK.K |
| K.YIIASGNLMADFR.A | ||||||
| R.ANLNAESQGR.L | ||||||
| R.EGDVVVPTTFPR.S | ||||||
| K.QQVSYDLFNFSR.G | ||||||
| K.ELEEREGDVVVPTTFPR.S |
a Band No. of proteins marked in Fig 1C and 1D
b Individual ions scores > 57 indicate identity or extensive homology (P < 0.05)